Peripheral blood mononuclear cells cryopreservation and transportation

PBMC cryopreservation and transportation

Abstract

Chimeric antigen receptor-T (CAR-T) cells have emerged as a treatment option for patients with hematologic malignancies. Usually, their manufacture has been carried out by specialized and centralized laboratories. A useful strategy, which allows a better logistical organization, is the peripheral blood mononuclear cells (PBMC) cryopreservation and transportation. This article aims to discuss the most relevant points related to the PBMC cryopreservation and transportation process. In addition, it proposes a kind of standard operational protocol (SOP) that can be very helpful for the cell processing labs.